In [39]: df
Out[39]: 
          0         1         2         3         4  mean
0  0.172742  0.915661  0.043387  0.712833  0.190717     1
1  0.128186  0.424771  0.590779  0.771080  0.617472     1
2  0.125709  0.085894  0.989798  0.829491  0.155563     1
3  0.742578  0.104061  0.299708  0.616751  0.951802     1
4  0.721118  0.528156  0.421360  0.105886  0.322311     1
5  0.900878  0.082047  0.224656  0.195162  0.736652     1
6  0.897832  0.558108  0.318016  0.586563  0.507564     1
7  0.027178  0.375183  0.930248  0.921786  0.337060     1
8  0.763028  0.182905  0.931756  0.110675  0.423398     1
9  0.848996  0.310562  0.140873  0.304561  0.417808     1

In [40]: df = df[['mean', 4,3,2,1]]