## set up some fake test data
time <- seq(0,72,12)
betagal.abs <- c(0.05,0.18,0.25,0.31,0.32,0.34,0.35)
cell.density <- c(0,1000,2000,3000,4000,5000,6000)

## add extra space to right margin of plot within frame
par(mar=c(5, 4, 4, 6) + 0.1)

## Plot first set of data and draw its axis
plot(time, betagal.abs, pch=16, axes=FALSE, ylim=c(0,1), xlab="", ylab="", 
   type="b",col="black", main="Mike's test data")
axis(2, ylim=c(0,1),col="black",las=1)  ## las=1 makes horizontal labels
mtext("Beta Gal Absorbance",side=2,line=2.5)
box()

## Allow a second plot on the same graph
par(new=TRUE)

## Plot the second plot and put axis scale on right
plot(time, cell.density, pch=15,  xlab="", ylab="", ylim=c(0,7000), 
    axes=FALSE, type="b", col="red")
## a little farther out (line=4) to make room for labels
mtext("Cell Density",side=4,col="red",line=4) 
axis(4, ylim=c(0,7000), col="red",col.axis="red",las=1)

## Draw the time axis
axis(1,pretty(range(time),10))
mtext("Time (Hours)",side=1,col="black",line=2.5)  

## Add Legend
legend("topleft",legend=c("Beta Gal","Cell Density"),
  text.col=c("black","red"),pch=c(16,15),col=c("black","red"))